Notch1 and HPV Genotyping in Cervical Adenocarcinomas
AC Policiano, P Alves, L Martins, A Felix. Faculdade de Medicina, Lisboa, Portugal; Instituto Portugues de Oncologia, IPOLFG, Lisboa, Portugal; IPOLFG e Faculdade de Ciências Médicas, Lisboa, Portugal
Background: The link between HPV infection and cervical carcinogenesis is well established. However, additional events are needed for tumor development. Several reports suggest that Notch signaling may be involved in neoplastic transformation and it can function as a promotor or suppressor of tumor growth. It is believed that in cervical carcinomas Notch signalling might contribute to the process of tumorigenesis in association with HPV. In this study we aim to examine Notch1 and HES1 immunoexpression and to genotype HPV in a series of adenocarcinomas of the uterine cervix.
Design: Notch1C-20(receptor); Notch1EC(extracellular domain); Notch IC(intracellular domain); Notch1-S3(cleaved) and HES1(Notch1 signaling transcription target) immunoexpression was evaluated in a tissue microarray constructed with representative regions from 41 endocervical adenocarcinomas and from 6 normal endocervical specimens. Patients files were reviewed and morphological features characterized. HPV detection was done using SPF-10 broad-spectrum primers PCR, subsequently followed by DEIA and genotyping by LiPA25. Samples for both techniques were obtained from formalin fixed and paraffin embedded tissues.
Results: The Notch proteins were highly expressed in adenocarcinomas of the cervix (Table 1) in contrast to normal endocervical glands (all 6 were negative with all antibodies and had no HPV DNA).
|in situ adc||4||2(50%)||1(25%)||4(100%)||4(100%)||4(100%)||2(50%)|