Expression of BDNF, TrkB and p53 in Uterine Cervical Cancer
A Moon, JY Lee, I Kang, SK Lee, YW Kim, YK Park, J Lee. Kyung Hee University, Seoul, Republic of Korea
Background: Cervical cancer is the 4th most common cancer in Korean women. The neurotrophic receptor tyrosine kinase B (TrkB) and its high affinity ligand brain-derived neurotrophic factor (BDNF) play critical role in the central and peripheral neural system. Recent reports showed that higher levels of BDNF and TrkB generally correlate with more aggressive tumor behavior and they are involved in tumor proliferation, invasion and metastasis in a variety of cancers. The tumor suppressor gene p53 is frequently mutated gene in human cancer. P53 has important role in cell cycle arrest, DNA repair activation and regulation of apoptosis. The aim of the present study was to examine the potential role of BDNF, TrkB and p53 in cervix cancer focusing on the correlation between the expression of each protein and clinicopathologic parameters.
Design: RT-PCR analysis for BDNF and TrkB mRNA expression was performed on not only cervix cancer cell lines (SiHa, CaSki, HeLa, ME180) but also on human tissues of squamous cell carcinoma and normal tissue of the uterine cervix. We also analyzed TrkB, BDNF and p53 expression using immunohistochemistry in 80 patients with invasive squamous cell carcinoma of cervix.
Results: Consistent over-expression of BDNF and TrkB mRNA was found in all of the cervix cancer cell lines and squamous cell carcinoma tissues of cervix, compared to the normal cervical tissues. In immunohistochemical stain, high expression of BDNF, TrkB and p53 was observed in 54 (67.5%), 35 (43.8%) and 31 (38.8%) cases in total 80 cases, respectively. High expression of BDNF was inversely correlated to depth of invasion (p=0.04), lymphatic invasion (p=0.026) and lymph node metastasis (p=0.009). High TrkB expression was associated with keratinizing squamous cell carcinoma (p=0.001). P53 expression was significantly related to the tumor stage (p=0.001), greatest dimension (p=0.002) and depth of invasion (p=0.005).
Conclusions: Our data demonstrated that BDNF and TrkB expression might have a significant role in tumorigenesis of uterine cervical squamous cell carcinoma. BDNF predicted a better prognostic value but, TrkB did not correlate with patient outcome. As expected, p53 was a useful poor prognostic marker in uterine cervical cancer.
Category: Gynecologic & Obstetrics
Tuesday, March 23, 2010 9:30 AM
Poster Session III # 179, Tuesday Morning