Expression of C-Kit, Platelet-Derived Growth Factor D and Platelet-Derived Growth Factor Receptor α in Ovarian Granulosa Cell Tumors
T Jazaerly, S Bandyopadhyay, A Semaan, M Ibrahim, B Albashiti, A Munkarah, M Raza, K Hayek, R Ali-Fehmi. Wayne State University, Detroit, MI; Henry Ford Health System, Detroit, MI; Karmanos Cancer Institute, Detroit, MI; St John Hospital, Detroit
Background: Granulosa cell tumors (GCTs) generally carry a good prognosis but with a small subset exhibiting a more aggressive clinical course including a relative insensitivity to chemotherapy. The aim of this study was to determine the potential prognostic significance of the immunohistochemical (IHC) expression of C-Kit, Platelet-derived growth factor D (PDGFD) and platelet derived growth factor receptor-α (PDGFR-α), in ovarian GCTs.
Design: We identified 30 consecutive ovarian GCTs resected at the Karmanos Cancer Center between 1999 and 2005. Clinical/surgical and detailed pathological data were available on all patients including age, tumor stage, capsule integrity and histologic type (juvenile vs. adult). Representative tumor sections were stained with antibodies to C-Kit, PDGFD and PDGFR-α. IHC intensity (graded as 1+, 2+, and 3+) and percentage of positive cells (with a cut off 30%) were used to determine high and low expression and were correlated with the clinicopathological parameters.
Results: The mean patient age at diagnosis was 48.23 years (19-88 years). Twenty five had stage I disease with 7 patients with capsule involvement, 4 patients were stage II and 1 patient was stage III. Twenty two patients, (73%) had adult type GCT tumors. PDGFD and PDGFR- α were seen in 66.7% and 53.3% of the tumors respectively. Of all the parameters investigated, high expression of PDGFD and PDGFR- α showed significant correlation with tumor size. Tumors< 5 cm in diameter had high expression of PDGFD and PDGFR- α in 28.5% and 33.5% of the tumors, while tumors > 5 cm in diameter had a high expression in 48% and 54% of the tumors respectively (P =0.04 and =0.02 respectively). There was no association between the expression of the proteins and patients' age, tumor stage, tumor rupture or histologic type. There was no immunoreactivity with C-Kit in any of the samples studied.
Conclusions: Our data indicate a high frequency of PDGFD and PDGFR- α expression in GCTs. We believe our study offers one of the first evaluation of the expression of PDGFD in GCTs and demonstrates a significant correlation between the size of the tumor and the expression of PDGFD and PDGFR- α.
Category: Gynecologic & Obstetrics
Monday, March 22, 2010 9:30 AM
Poster Session I Stowell-Orbison/Surgical Pathology/Autopsy Awards Poster Session # 157, Monday Morning