Proliferative Activity of Non-Fimbriated-End Fallopian Tube Epithelium in Women with and without High Grade Serous Carcinoma of Ovary/Peritoneum
J Hemminger, J Hurt, J Stephens, S Barsky, K Yearsley, A Suarez. The Ohio State University Medical Center, Columbus, OH
Background: Until recently, the fallopian tube (FT) of women diagnosed with high grade serous carcinoma of the ovary/peritoneum (HGSC) has been superficially examined by pathologists and excluded from staging systems and models of HGSC carcinogenesis. But putative precursor lesions of HGSC have now been identified in the fimbriated end of FT from women with BRCA mutations undergoing risk reducing salpingo-oophorectomy. However, non-fimbriated-end FT archival material is much more abundant and includes histologically identical epithelium, presumably exposed to similar field effects. Hence, we compared, using immunohistochemistry and digital image analysis, the proliferative activity of non-fimbriated-end FT epithelium in women with and without HGSC.
Design: Tissue microarrays were constructed with 2mm cores of uninvolved FT from 72 patients with HGSC (group 1) and 72 age-matched controls (+/- 2 years) without gynecologic cancer (group 2). Immunohistochemistry for Ki67 was performed on both tissue microarrays and staining quantification was determined with digital image analysis (TissueMine ®). Results were confirmed by light microscopy and technically poor cores were excluded. 49 FT pairs were considered adequate and used for further analysis.
Results: Both groups had almost identical ages (group 1: mean 61.39, std dev 12.83; group 2: mean 60.78, std dev 12.07; p value=0.808). The fallopian tubes associated with HGSC demonstrated a mean Ki67 proliferative index of 1.38% (std dev 1, range 0.19-4.39). Age-matched controls demonstrated a mean Ki67 proliferative index of 1.7% (std dev 1.83, range 0.02-8.85%). Thus, the proliferative activity was similar between these two groups (p value=0.857). Also, women ≤55 years of age had a mean proliferative index of 1.34 (std dev 1.02, range 0.21-3.95) and women > 55 had a mean proliferative index of 1.4 (std dev 1.01, range 0.19-4.39), p= 0.832.
Conclusions: Proliferative activity, represented by Ki67 nuclear immunoreactivity, of non-fimbriated-end fallopian tube epithelium is similar in patients with HGSC and age-matched controls. The proliferative index in both groups was low (average, 1.54%) and was not affected by the patient age. Further studies, with molecular and immunophenotypic tools, are necessary to evaluate the merits of non-fimbriated-end FT material in HGSC research.
Category: Gynecologic & Obstetrics
Tuesday, March 23, 2010 1:00 PM
Poster Session IV # 141, Tuesday Afternoon