[1062] Loss of Heterozygosity (LOH) and Immunohistochemical Analysis (IHC) of a Subset of Primary Fallopian Tube Carcinomas (PFTC) Arising in the Background of Tubal Intraepithelial Carcinoma "TIC” from Primary Peritoneal Serous Carcinomas with/without Associated Tubal Intraepithelial Carcinoma "TIC”
M Chivukula, R Edwards, M Nikiforova, G Mantha, K McManus, LA Niemeier. Magee Women's Hospital of UPMC, Pittsburgh, PA; Magee Women's Hospital of UPMC, Pittsburgh
Background: Recent literature is emerging on role of TIC as a precursor lesion of primary peritoneal serous carcinoma (PPSC) {Crum et al}.PAX2 is a developmental transcription factor expressed in wolffian and müllerian ducts has been demonstrated in serous carcinomas of the ovary (up to 60%). The role of PAX2 in PFTC /PPSC is yet to be defined. The aim of our study was to evaluate the expression of p53,PAX2, WT-1 both by LOH and IHC studies.
Design: Ten (10) cases of PFTC with TIC (Group 1), PPSC without associated TIC (PPSC/-) (Group 2) two (2) cases of PPSC with associated TIC (PPSC/+) (Group 3) were secured from the case files of MWH. p53 PAX2, WT-1 (nuclear stains) were scored as positive and negative. All tumors and corresponding normals were manually microdissected. A Panel of 6 polymorphic microsatellite markers corresponding to TP53, PTEN, and WT1 tumor suppressor genes were studied.
| PTEN/PAX 2 | P53 | WT1 | ||||
| LOH | IHC | LOH | IHC | LOH | IHC | |
| Group 1 | LOH 50% (5/10) | 100% (10/10) | 60% (6/10) | 100% (10/10) | 20% (2/10) | 100% (10/10) |
| Adjacent TIC | LOH 30% (3/10) | 100% (10/10) | 20% (2/10) | 20% (2/10) | No LOH 0% (10/10) | 0% (0/10) |
| Group 3 | No LOH 0% (0/2) | 50% (5/10) | No LOH 0% (0/2) | 100% (2/2) | No LOH 0% (0/2) | 100% (2/2) |
| Associated TIC | No LOH 0% (0/2) | 50% (5/10) | No LOH 0% (0/2) | 100% (2/2) | No LOH 0% (0/2) | 100% (2/2) |
| Group 2 | LOH 50% (5/10) | 80% (8/10) | 60% (6/10) | 100% (10/10) | 20% (2/10) | 100% (10/10) |