[1062] Loss of Heterozygosity (LOH) and Immunohistochemical Analysis (IHC) of a Subset of Primary Fallopian Tube Carcinomas (PFTC) Arising in the Background of Tubal Intraepithelial Carcinoma "TIC” from Primary Peritoneal Serous Carcinomas with/without Associated Tubal Intraepithelial Carcinoma "TIC”

M Chivukula, R Edwards, M Nikiforova, G Mantha, K McManus, LA Niemeier. Magee Women's Hospital of UPMC, Pittsburgh, PA; Magee Women's Hospital of UPMC, Pittsburgh

Background: Recent literature is emerging on role of TIC as a precursor lesion of primary peritoneal serous carcinoma (PPSC) {Crum et al}.PAX2 is a developmental transcription factor expressed in wolffian and müllerian ducts has been demonstrated in serous carcinomas of the ovary (up to 60%). The role of PAX2 in PFTC /PPSC is yet to be defined. The aim of our study was to evaluate the expression of p53,PAX2, WT-1 both by LOH and IHC studies.
Design: Ten (10) cases of PFTC with TIC (Group 1), PPSC without associated TIC (PPSC/-) (Group 2) two (2) cases of PPSC with associated TIC (PPSC/+) (Group 3) were secured from the case files of MWH. p53 PAX2, WT-1 (nuclear stains) were scored as positive and negative. All tumors and corresponding normals were manually microdissected. A Panel of 6 polymorphic microsatellite markers corresponding to TP53, PTEN, and WT1 tumor suppressor genes were studied.

PTEN/PAX 2P53WT1
LOHIHCLOHIHCLOHIHC
Group 1LOH 50% (5/10)100% (10/10)60% (6/10)100% (10/10)20% (2/10)100% (10/10)
Adjacent TICLOH 30% (3/10)100% (10/10)20% (2/10)20% (2/10)No LOH 0% (10/10)0% (0/10)
Group 3No LOH 0% (0/2)50% (5/10)No LOH 0% (0/2)100% (2/2)No LOH 0% (0/2)100% (2/2)
Associated TICNo LOH 0% (0/2)50% (5/10)No LOH 0% (0/2)100% (2/2)No LOH 0% (0/2)100% (2/2)
Group 2LOH 50% (5/10)80% (8/10)60% (6/10)100% (10/10)20% (2/10)100% (10/10)



Results: [Table1] shows a summary of LOH and IHC of all groups.
Conclusions: There was high frequency of LOH for the PTEN/PAX 2 and p53 genes in all groups. Group 2 and Group 3 exhibits different LOH patterns. Group 1 shows similar LOH patterns to Group 2. TIC of PFTC shows a different pattern in comparison to associated TIC.
Category: Gynecologic & Obstetrics

Tuesday, March 23, 2010 8:45 AM

Platform Session: Section D, Tuesday Morning

 

Close Window