Evaluation of the Combined Utility of p16 and ProEx C Immunohistochemistry in Distinguishing Endocervical Glandular Neoplasia from Its Benign Glandular Mimics
N Banet, C Livasy. Univ of North Carolina, Chapel Hill, NC
Background: Cervical adenocarcinoma accounts for 15-25% of all cervical cancers. Identification of endocervical glandular neoplasia (EGN) in cervical specimens may be difficult due to small sample size and/or the presence of benign glandular mimics. Proteins p16, topoisomerase II alpha, and MMP2 have been shown to be upregulated in HPV-associated neoplasia. The aim of this study was to evaluate the combined utility of p16 and ProEx C in distinguishing EGN from its benign glandular mimics.
Design: Patients were selected retrospectively including those with endocervical adenocarcinoma in situ (AIS), usual-type invasive endocervical adenocarcinoma and several benign glandular mimics. Representative sections from each case were selected for immunohistochemical (IHC) studies for p16 and ProEx C. The IHC slides were reviewed by two pathologists and scored for intensity (0-3) and percent staining.
|Normal endocervix (n=8)||2||6||0||0||0|
|Tubal metaplasia (n=10)||2||2||0||5||1|
|Inflammatory atypia (n=8)||5||3||0||0||0|
|Microglandular hyperplasia (n=8)||8||0||0||0||0|
|AIS (n= 11)||0||0||0||0||11|
|Invasive adenocarcinoma (n= 9)||1||0||0||0||8|
|Normal endocervix (n= 8)||1||7||0||0||0|
|Tubal metaplasia (n=10)||1||7||1||1||0|
|Inflammatory atypia (n=8)||1||7||0||0||0|
|Microglandular hyperplasia (n=8)||2||5||0||1||0|
|Invasive adenocarcinoma (n=9)||0||0||0||1||8|