EPLIN Expression in Primary and Metastatic Prostatic Adenocarcinoma: A Tissue Microarray Analysis of 102 Patients
D Wu, S Zhang, CS Moreno, AO Osunkoya. Emory University School of Medicine, Atlanta, GA
Background: Epithelial protein lost in neoplasm (EPLIN) is a novel cytoskeletal protein that is preferentially expressed in human epithelial cells. Though initially thought to have decreased expression in neoplastic tissue due to transcriptionally down-regulated genes in cancer cells, the role of EPLIN in prostate cancer progression and metastasis remains unclear.
Design: A tissue microarray composed of tissue cores from 102 patients with PCa was constructed. 5 of the 102 patients that had metastatic PCa to lymph nodes were also identified. Immunohistochemical stains were performed for EPLIN in both the TMA and sections from the patients' lymph node metastases. Intensity of tissue expression was graded as 0, 1+, 2+, 3+. Statistical analysis was performed to determine the significance of EPLIN expression based on Gleason scores, and in primary versus metastatic PCa.
Results: The Gleason scores in the 102 patients were as follows: Gleason score 6 (39 patients), Gleason score 7 (38 patients), Gleason score 8 (21 patients) and Gleason score 9 (4 patients). Comparison of EPLIN expression in patients with the various Gleason scores were as follows; Gleason score 6 vs 7 (p=0.38), Gleason score 7 vs 8/9 (p=0.04) and Gleason score 6 vs 8/9 (p=0.002) Figure 1. Patients with metastatic PCa to lymph nodes had Gleason scores of 7 (1 patient), 8 (3 patients) and 9 (1 patient). Comparison of EPLIN expression in primary versus metastatic PCa cases showed slightly decreased EPLIN expression in the metastatic tumors: Gleason score 7 (primary 2+, metastasis 1+), Gleason score 8 (primary 3+, metastasis 2+) and Gleason score 9 (primary 3+, metastasis 2+).
Conclusions: EPLIN expression increases with Gleason score, with more statistically significant differential expression between well differentiated and poorly differentiated PCa. EPLIN expression appears to be down regulated in metastatic PCa. It is highly conceivable that EPLIN may play a role in PCa progression and epithelial-to-mesenchymal transition in metastatic PCa.
Category: Genitourinary (including renal tumors)
Wednesday, March 24, 2010 1:00 PM
Poster Session VI # 121, Wednesday Afternoon