A Next Generation HPV Test That Simultaneously Detects, Types, and Quantifies All 15 High Risk HPVs Type and Distribution of HPV in Cervical Pap Smears
P Manna, S Kerley, R Garrison, D Stark, T Parrish, P Clark, G Scholtz, MR Quddus, S Bose. Physicians Reference Laboratory, Overland Park, KS; Women & Infants Hospital, Providence, RI; Cedars-Sinai Medical Center, Los Angeles, CA
Background: Reflex high risk (HR) HPV testing is the preferred approach for managing patients with atypical cervical cytology. However, many HPV tests neither detect all 15 HR HPV types nor type them simultaneously. Typing is important to determine both persistent infection and oncogenic potential. While both HPV16 and 56 are HR types, the risk of developing cervical cancer by HPV16 is significantly higher than HPV56 (434x Vs 45x). Thus, complete and type-specific HPV detection increases the sensitivity and specificity of the test and helps clinicians assess the risk for cervical cancer. We have developed and validated a type-specific HPV test and applied on patients with atypical cervical cytology.
Design: COMPLeTe Care HPV test simultaneously detects, types, and quantifies all 15 HR HPV types with an internal control for sample DNA. This complex multiplex real time PCR test has been extensively validated and then utilized as a reflex HPV test on 2206 patients with atypical cervical cytology. This report describes the distribution of type specific HPV in these samples.
Conclusions: HPV type specific testing with COMPLeTe Care revealed a significant number of patients with multiple infections. HPV16 was the most prevalent type. Surprisingly, the prevalence of HPV18 was significantly less than other types. This next generation test has many advantages and sets a new standard in cervical cancer screening that will improve patient management and critically reduce morbidity, mortality, and costs.
Wednesday, March 11, 2009 9:30 AM
Poster Session V # 29, Wednesday Morning