Are High Risk HPV Types 73 and 82 Being Overlooked in Cervical Cancer Screening?
P Manna, S Kerley. Physicians Reference Laboratory, Overland Park, KS
Background: Individual viruses of the 100-plus members of the HPV family are defined by DNA sequence homologies that induce type-specific pathologies. Epidemiologic classification, based on 11 case-controlled studies identified 15 HPV types as high-risk types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 73, and 82) for cervical cancer. Furthermore, the prevalence of high risk HPV types varies with the geographic locations. However, many diagnostic tests do not routinely detect HPV types 73 and 82. Here, we determined the prevalence and distribution of HPV types 73 and 82 utilizing a next generation HPV test that simultaneously detects, types, and quantifies all 15 high risk HPV types.
Design: COMPLeTe Care HPV test simultaneously detects, types, and quantifies all 15 high risk HPV types that are known to cause cervical cancer. The test also detects beta globin as an internal control. This complex multiplex real time PCR test was extensively validated and compared with other methods. The test was then utilized on 2913 patients to determine the prevalence and distribution of HPV types 73 and 82. The majority of this patient population had abnormal Pap smears.
Results: The prevalence of high risk HPV types 73 and 82 is significantly higher in patients with abnormal Pap smears than in patients with normal Pap smears.
Conclusions: Analysis from numerous studies unanimously concluded that 15 HPV types are considered as high risk because of their importance in cervical cancer development. Here, we have shown the prevalence and distribution of HPV types 73 and 82, the two often neglected high risk HPV types in screening test. Our studies justified the importance of adding these two high risk HPV types in routine screening test as they were not only present in patients with ASCUS Pap smears but also in patients with low and high grade Pap smears.
Wednesday, March 11, 2009 9:30 AM
Poster Session V # 24, Wednesday Morning