Cell Chip Platform Using Nanomesh and Collagen Coated-PET Discs in Cervicovaginal Cytology
JS Jang, YJ Choi, CM Moon, JY Park, SH Lee, HI Bae. Kyungpook National University School of Medicine, Daegu, Korea
Background: Adhesion of cells on surface is an important factor in cytology smear. According to development of nanotechnology, biocompatible nanofibers have been studied and many biomedical applications have been widely reported. Poly (3-hydroxybutyrte-co-3-hydroxyvalerate) (PHBV) is one of the most promising materials for tissue engineering. Composite solution of PHBV and the natural calf collagen peptide (PHCP), dissolved in 2 wt% 2,2,2-trifluroethanol (TTF), were electrospun on polyethylene teraphthalate (PET) film (nanomesh plate). Nanomesh plates and type I collagen-coated PET discs attached to a 10-holed slide (cell chip frame), using for high throughput screening and immune- and special staining of cervicovaginal cytology.
Design: We used remnant cervicovaginal cells in ThinPrep solution (Cytyc, MA, USA). After centrifuged and mixed with LiquiPrep (LGM international, FL, USA) solution, the cells were smeared on the representative cell chip. Among 19 epithelial abnormal specimens above ASCUS, 8 NILM specimens for negative control were arrayed in cell chips. Each specimen was smeared on nanomesh, collagen coated and conventional slide cell chips. Each cell chip had a HeLa cell line sample for positive control. Total 24 cell chips, including 6 nanomesh cell chips, 9 collagen coated slide cell chips and 9 conventional slide cell chips were made. The cell chips were respectively performed Papanicolaou stain, Ki-67 and p16 immunocytochemical stain. Namomesh cell chips were excluded in p16 immunochemical stain for problems in cell adhesion. Each of 10 samples were interpreted on one cell chip and compared with cytologic and final histologic diagnosis.
Results: Among total 57 tested disc, 21 samples were in agreement with conventional diagnosis, 20 samples showed category B and C disagreement, 16 samples were insufficient for diagnosis. Sensitivity of p16 for HSIL and squamous cell carcinoma was 66.6% and specificity was 81%. In Ki67, sensitivity was 58.3% and specificity was 51.7%.
Conclusions: Even though, cell chip had some defects, such as cellular overlapping and air bubbles on nucleus according to technologist, relationship between p16 and epithelial abnormality of cervical cytology, reported in literatures was well reproved in cell chip platform. The current results indicate that cell chip prepared from residual Thinprep material should be an economic, and high throughput adjuvant test platform with high reproducibility of cervicovaginal samples.
Wednesday, March 11, 2009 9:30 AM
Poster Session V # 26, Wednesday Morning