Comparison of Desmoplastic Reaction and Invading Ability in Invasive Ductal Carcinoma (IDC) of Breast and Prostatic Adenocarcinoma Based on Expressions of Hsp47 and Fascin
AR Kandiloglu, N Nese, G Simsek, M Lekili, A Ozdamar, A Catalkaya, T Coskun. Celal Bayar University, Manisa, Turkey
Background: IDC is characterized by prominent desmoplasia, whereas conventional PCa generally have little/no desmoplastic reaction. HSP47 is major collagen specific chaperone responsible for formation and maturation of collagen during protein synthesis. Fascin, an actin binding protein, plays important role in cancer invasion and metastasis. Both these proteins are overexpressed by various carcinomas. We investigated the diversity within IDC and PCa by evaluating immunohistochemical expressions of HSP47 and fascin, the molecules which are related to desmoplasia and invasion, and analyzed its correlation with clinicopathological parameters.
Design: Forty nine mastectomies and 57 radical prostatectomies were immunostained with HSP47 and fascin. 10 mammoplasty specimens and 10 prostatectomy specimens performed for prostate hyperplasia were chosen as control group. Immunoreactivity (IR) was evaluated as: 0: <5%, 1+: 5-25%, 2+: 25-50%, 3+: >50%. Desmoplasia was designated as 0 to 3.
Results: HSP47 and fascin were localized to cytoplasm and were significantly more often expressed in both the tumors than control groups (p<0.05). PCa demonstrated immunoreactivity to HSP47 significantly more often than IDC. Tumor size correlated with fascin expression in IDC (p<0.05). HSP47 expression was noted significantly more often in PCa involving both left and right lobes.
Clinicopathologic Parameters and Immunohistochemical Evaluation
|Mean age||55 years||67 years|
|Mean tumor size||3.3 cm||Mean PSA level||12.7 ngr/dl|
|Poorly-differantiated tumor||12.2%||Gleason score 8-10||19.3%|
|c-erbB-2 (2+/3+) score||33%||Extraprostatic extension||29.8%|
|ER negativity||26.5%||Seminal vesicle involvement||15.8%|
|PR negativity||32.7%||Involvement of both right and left lobes||88%|
|Axillary LN positivity||69%||Recurrence||11.6%|
|Mean follow-up||42 months (64%)||52 months (75%)|
|Dead of disease||20.0%||4.7%|
|HSP47 (2+ and 3+ IR)||29%||42%|
|HSP47 in control cases (n=10)||0%||20%|
|Fascin (2+ and 3+ IR)||65%||23%|
|Fascin in control cases (n=10)||0%||0%|
Conclusions: HSP47 and fascin expression may play role in the pathogenesis of IDC and PCa because their expression is significantly higher in IDC and PCa than their normal counterpart. Although there is no relationship with recurrence or metastatic status, fascin overexpression correlated with tumor size which may prompt its use as a prognostic factor in IDC.
Wednesday, March 11, 2009 1:00 PM
Poster Session VI # 235, Wednesday Afternoon