Aberrant Co-Expression of D2-40 and CD31/CD34 in Vascular Neoplasm Is an Indicator of Aggressive Behavior
T Maruno, T Tanaka, MD Hofer, Y Iinuma, K Nakagawa, T Hori, J Fukuoka. Toyama University Hospital, Toyama, Japan; Brigham and Women's Hospital, Boston
Background: Recent recognition of lymphatic vascular markers has unveiled biological events in the formation of lymphatic and blood vessel network. Yet, their expression in various vascular tumors are not carefully observed. Understanding the expression profiles and interactions of those markers in vascular tumor is important which may lead to a better understanding of lymphangiogenesis and angiogenesis in various diseases.
Design: A Tissue microarray was constructed from 40 hemangioma, 8 lymphangioma, 13 pyogenic granuloma, 3 endothelioma, and 4 angiosarcoma cases with duplicated cores. We analyzed the expression of D2-40, CD31 and CD34 with immunohistochemistry using consecutive TMA slides. Staining results were scanned by a virtual slide system (VS-100, Olympus), and the expression compared among the slides.
Results: Cells with positive D2-40 staining were observed in 55% of hemangioma, 100% of lymphangioma, 38% of pyogenic granuloma, 67% of endothelioma, and 100% of angiosarcoma. The neoplastic vessels with CD34 expression were found in all except two lymphangioma cases and one hemangioma case. Remarkable co-expression of D2-40 & CD31 was frequently found in lymphangioma (38%(3/8)), endothelioma; (33% (1/3)), and angiosarcoma (100% (4/4)), and rarely in hemangioma (5% (2/40) and pyogenic granuloma (7.2% (1/13)). Similarly, high co-expression of D2-40 and CD34 was also found in the same tumors. Especially, three out of four cases of angiosarcoma and two out of three cases of endothelioma showed relatively diffuse and strong co-expression in D2-40 and CD31.
|Expression(%/positive cases)||Coexpression(%/positive cases)|
Conclusions: Our results indicate that the aberrant co-expression of D2-40/CD31 or D2-40/CD34 are a common event in lymphangioma, endothelioma, and angiosarcoma. In the tumor of blood vessel, excluding lymphatic vessels, aberrant expression can be an indicator of aggressive nature, and may also be useful diagnostic marker.
Category: Special Category for 2009 - Pan-genomic/Pan-proteomic approaches to Cancer
Monday, March 9, 2009 9:30 AM
Poster Session I Stowell-Orbison/Autopsy Award # 241, Monday Morning