High Levels of Expression of Human Stromal Cell-Derived Factor-1 (SDF-1) Are Associated with Worse Prognosis in Patients with Pancreatic Ductal Adenocarcinoma
J Liang, S Zhu, R Bruggeman, D Evans, R Zaino, D Zander, H Wang. Penn State Hershey Medical Center, Hershey, PA; U.T. M.D Anderson Cancer Center, Houston, TX
Background: Recently, stromal cell-derived factor-1 (SDF-1, CXCL12), together with its specific receptor, CXCR4, have been shown to mediate invasiveness and metastatic behavior in a number of cancers, including ovarian, prostate, bladder, breast and pancreatic cancers. The expression and significance of SDF-1 in human pancreatic ductal adenocarcinoma (PDA) have not been studied in detail.
Design: We prepared a tissue microarray consisting of 1.0-mm cores of tumor (two cores per tumor) and paired non-neoplastic pancreatic tissues from 72 pancreaticoduodenectomy specimens of PDA. Cytoplasmic expression of SDF-1 was evaluated by immunohistochemistry with mouse anti-human SDF-1/CXCL12 antibody (dilution 1:6000). The staining results were categorized as SDF-1-high (H) (strong cytoplasmic staining of 10% of tumor cells) or SDF-1-low (L) (no staining or weak staining of <10% of tumor cells). Statistical analyses were performed using SPSS software and survival was evaluated by Kaplan-Meier log-rank test.
Results: 25/72 (35%) PDAs demonstrated high levels of SDF-1 expression . The median overall and disease-free survival rates for patients with SDF-1-H PDAs were 26.1 and 11.1 months, respectively, compared to 44.3 and 22.3 months for patients with SDF-1-L tumors (log-rank test, p=0.047 and p=0.021). High SDF-1 expression was associated with poor overall and disease-free survival in univariate analysis (p=0.051 and p=0.024). In multivariate analysis, high SDF-1 expression was significantly correlated with poor overall and disease-free survival (p=0.019 and p=0.02) independent of age, gender, tumor size and differentiation.
Conclusions: High levels of SDF-1 expression are associated with poor overall and disease-free survivals in patients with PDA. SDF-1 may serve as a useful independent prognostic marker for PDA.
Category: Liver & Pancreas
Wednesday, March 11, 2009 9:30 AM
Poster Session V # 233, Wednesday Morning