Clinicopathologic Characteristics in Correlation with Protein Expression Profile in Triple Negative Breast Cancer in Our Population
R Busch, C Cohen, SE Pambuccian, R Gamez, R O'Regan, L Yang, HE Gulbahce, GM Oprea-Ilies. Emory University, Atlanta, GA; University of Minnesota, Minneapolis, MN
Background: Triple negative tumors (TNT) of the breast are ER, PR and Her negative and represent a group of tumors with no benefits from specific therapy. They are more frequent in younger women and portend a worse outcome. We studied clinico-pathological features and markers associated with the triple negative phenotype.
Design: Tissue microarray (TMAs) from 125 breast carcinomas diagnosed during a 6 years period. Only tumors that did not show staining for ER, PR and were scored as 0, 1 or 2 with FISH confirmation for non-amplified Her2 were included. Immunohistochemical (IHC) staining was performed for Cytokeratin (CK) 5/6, 7,8,14 18, 19, Vimentin, CD44, Topoisomerase 2, Survivin, c-Kit, p53, p63, androgen receptor (AR) and Zeb. Clinical pathologic data, including race, age, tumor size, tumor grade, TNM stage and follow-up was obtained. Statistical analysis was performed using chi square analysis and linear regression with a p value of 0.05 or less significant.
Results: Of the 135 patients, 107 were African-American (AA), 18 Caucasians (CS), 6 other and 4 unknown. We further analyzed the data for the AA and CS groups. The age, pathology and the IHC statistically significant data are presented in table 1.
* Denotes statistical significance. All other IHC stains did not show statistical significance by chi square
|African-Americans 107 (85.6%)||Caucasians 18 (14.4%)|
|Age less or 50-years|
|Age older than 50-year|
In addition CD44 and EGFR were more common in the AA population. CK7, p-cadherin and race were predictive of T stage. CK14 and survivin were predictive for N stage.
Conclusions: 1. In our population we found no statistically significant inter-racial age difference. 2. In regards to TNM elements, there was a statistically significant difference in the tumor size, while such a difference was not noted in lymph node status. 3. Within the large panel of IHC we performed, statistical differences were found in CD44 and EGFR expression, both more frequently found in the AA population. As CD44 is a putative marker for breast stem cells, further investigation is warranted in this direction.
Tuesday, March 10, 2009 1:00 PM
Poster Session IV # 37, Tuesday Afternoon