Immunohistochemical Expression of LYVE-1 Is a Prognostic Factor in Ovarian Carcinoma
A Marino-Enriquez, M Mendiola, J Barriuso, A Redondo, G Hernandez-Cortes, E Perez-Fernandez, A Suarez, D Hardisson. Hosp Univ La Paz, Madrid, Spain
Background: Lymphangiogenesis and lymphatic density correlate with prognosis in several malignancies including melanoma, colorectal and lung cancer, but their significance in ovarian carcinoma (OC) is unclear. Expression of lymphatic markers in non-lymphatic cells has been reported but is not well understood.
Design: We studied 68 patients with OC treated at our Hospital between 1996 and 2003. All the patients were homogeneously treated by debulking surgery and a platinum/taxane-based chemotherapy. Immunohistochemistry was performed on tissue microarrays. We examined the expression of the vascular markers CD34, LYVE-1 and D240 in tumor cells (0/1) and the microvascular (MVD) and lymphovascular density (LVD) as detected respectively by CD34 and D240 positive vessels in tumor stroma (0-3). Expression values were correlated with clinicopathological variables, including progression-free (PFS) and overall survival (OS).
Results: Clinicopathological data and results of the study are summarized in Table 1. On univariate analysis, expression of LYVE-1 in tumor cells was significantly associated with worse OS (Figure 1). No association was found between MVD, LVD or the expression of CD34 and D240 in tumor cells with any clinicopathological variables.
|Age (mean, range)||55.19 (21-82) yrs|
|Histology (n)||Serous (43), others (25)|
|Grade (n)||1 (5), 2 (26), 3 (37)|
|Stage (n)||I (2),II (5),III (51),IV (10)|
|PFS (mean, range)||19.58 (1.37-67.00) mo|
|OS (mean, range)||46.57 (1.57-127.47) mo|
|CD34 (n)||0 (67), 1 (1)|
|MVD [CD34+ vessels](n)||0 (5), 1 (32), 2 (23), 3 (3)|
|LYVE-1 expression (n)||0 (38), 1 (28)|
|D240 expression (n)||0 (35), 1 (31)|
|LVD [D240+ vessels] (n)||0 (16), 1 (18), 2 (22), 3 (10)|
Conclusions: In our series, the expression of LYVE-1 on tumor cells significantly correlates with worse OS in OC. MVD and LVD do not have impact in PFS or OS. Further studies are needed to clarify the significance of the expression of lymphatic markers in tumor cells in OC. Grants: FIS PI07/0651 and Fundacin Mutua Madrilea.
Wednesday, March 11, 2009 9:30 AM
Poster Session V # 159, Wednesday Morning