The Distribution of HPV Types in Invasive Cervical Carcinoma. Analysis of 9760 Cases
B Lloveras, O Clavero, M Alejo, J Ordi, S de Sanjose, J Klaustermeier, W Quint, JP Brunsveld, N Guimera, L Alemany, S Garland, A Nessa, YL Qiao, M Grce, C Clavel, L Lombardi, A Ferrera, N Bhatla, A Jain, L Mariani, T Sasawaga, C Menendez, K Banjo, EJ Domingo, CY Chou, S Chichareon, A Usubutun, E Oliva, TC Wright, V Garcia, G Sanchez, N Munoz, FX Bosch. Institut Catala d'Oncologia, L'Hospitalet, Barcelona, Spain; Hospital Clinic, Barcelona, Spain; Delft Diagnostic Laboratory, Voorburg, Netherlands; Hospital del Mar, Barcelona, Spain; Fully Displayed at Presentation
Background: Cervical cancer is caused by HPV infection, most frequently by high risk HPV types 16 and 18. However, the distribution of other less frequent types is not well described and may be important to predict the impact of first generation vaccines.
Design: The objective was to describe the HPV genotype distribution in invasive cervical cancer worldwide. Paraffin embedded cervical cancer cases were collected (from 1920 to 2005). HPV detection was done by SPF-10 broad-spectrum primers PCR subsequently followed by DEIA and genotyping by LiPA25 (version 1). Samples were tested at HPV laboratories at ICO (Barcelona, Spain) and at DDL (The Netherlands).
Results: Of 13,239 cases included in the study, 11,171 have now been evaluated and 9,760 considered suitable for testing. Histologically, 89% were squamous cell carcinomas, 8% adenocarcinomas, 1.4% adenosquamous carcinomas and 1.6% other subtypes. HPV genotype data are available for 8,785 cases. The five most common types detected were HPV 16 (60.3%), HPV 18 (10.3%), HPV 45 (5.9%), HPV 31 (4.2%) and HPV 33 (4.0%). This distribution was consistent across continents with the exception of Asia where HPV 58 ranked 3rd and in Oceania where HPV 68/73 ranked 4th. Among adenocarcinomas, HPV 16 accounted for 47.8% (range by continent= 35.5%-53.2%) and HPV 18 for 29.0% (range by continent= 23.5%-36.9%). Presence of exclusively low risk types accounted for 0.44% of all cases. Multiple infections were detected in 4.8% of the samples.
Conclusions: HPV types 16, 18, 31, 33 and 45 were the most common types in cervical cancer cases around the world, accounting for 85% of all the cases. There were no statistically significant differences in the distribution of these types over time (p>0.05).
Monday, March 9, 2009 1:00 PM
Poster Session II # 141, Monday Afternoon