Prognostic Implication of Immunohistochemical Runx2 Expression in Osteosarcoma
KY Won, YW Kim, HR Park, YK Park. Kyung Hee University, Seoul, Republic of Korea; Hallym University, Seoul, Republic of Korea
Background: Osteosarcoma is the most common primary bone malignancy. Many genetic markers have proven to have prognostic value in osteosarcoma and studies are underway to determine their potential applications as specific therapeutic targets. Runx2, Indian hedgehog (IHH), and Sox9 are proteins that play major roles in bone formation and tumorigenesis. We studied the protein expression of Runx2, IHH, and Sox9 in osteosarcoma and correlated their expression with clinicopathological variables. We also studied the prognostic value of the expression of these three genes in osteosarcoma.
Design: We produced 48 formalin-fixed, paraffin-embedded tissue microarrays containing osteosarcoma tissue cores for immunohistochemical staining of Runx2, IHH and Sox9. We evaluated the expression of each gene by immunohistochemical staining, and analyzed the relationship between expression and clinicopathological parameters.
Results: The high expression of Runx2 was significantly related to metastasis (p=0.015). The high expression of Runx2 indicated a trend toward a poor survival rate (p=0.056). The high expression of IHH and Sox9 were not related to any clinicopathological parameters.
Conclusions: The high expression of Runx2 was significantly related to tumor metastasis in osteosarcoma. Our results suggest that overexpression of Runx2 might be a useful prognostic marker in osteosarcoma cases.
Category: Bone & Soft Tissue
Tuesday, March 10, 2009 9:30 AM
Poster Session III # 19, Tuesday Morning