[2957.793] Effects of E-cigarette Exposure on Early Postnatal Alveolar Development in Neonatal Mice
Madoka Hayashi, Gang Chen, Joseph M. Collaco, Sharon A. McGrath-Morrow. Eudowood Division of Pediatric Respiratory Sciences, Johns Hopkins School of Medicine, Baltimore, MD; Department of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA.
BACKGROUND: In utero and/or postnatal cigarette smoke exposure can increase the risk of small airway dysfunction and worsen asthma severity in early childhood. Recently the use of e-cigarettes has gained popularity however the health effects of e-cigarette exposure on the lungs of young children are unknown. In particular there is no data on whether exposure to e-cigarette smoke has a detrimental effect on postnatal lung growth, as previously described with tobacco smoke.
OBJECTIVE: The goal of this study was to determine if daily exposure to e-cigarette smoke in neonatal mice can lead to significant systemic nicotine absorption and inhibition of postnatal lung growth during a critical period of postnatal lung development.
DESIGN/METHODS: One day old mice were divided into three groups. Two groups were exposed to e-cigarettes containing either 1.8% nicotine or 0% nicotine for 2hrs/day. Age-matched control siblings were kept in room air. Following a ten day exposure, plasma and urine nicotine and cotinine levels were measured from the neonatal mice, alveolar growth was assessed by mean linear intercept (MLI) measurements, cell counts were obtained from bronchoalveolar lavage and gene expression was quantified by real-time PCR using whole lung homogenate.
RESULTS: Plasma and urine nicotine and cotinine levels were found to be significantly higher in neonatal mice exposed to 1.8% nicotine smoke compared to the other groups (p<0.01). MLI measurements were also significantly larger in the mice exposed to 1.8% nicotine compared to the other 2 groups (p<0.001) indicating impairment in alveolar growth. There were minimal differences in BAL cell count and gene expression between all groups.
CONCLUSIONS: Daily exposure to e-cigarettes containing 1.8% nicotine was associated with significantly elevated plasma and urine nicotine and cotinine levels and impaired alveolar growth in neonatal mice. These findings suggests that exposure to e-cigarette smoke may cause significant systemic nicotine absorption in young children and may adversely affect postnatal alveolar growth.
First Author is a Fellow in Training
Session: Poster Session: Pulmonology (4:15 PM - 7:30 PM)
Date/Time: Sunday, May 4, 2014 - 4:15 PM
Room: Exhibit Hall C - Vancouver Convention Centre
Course Code: 2957