[2008] [FRI0245] COLLAGEN DEGRADATION PRODUCTS AND ELASTIN IN SYSTEMIC AND LOCALIZED SCLERODERMA
R. Becvar1, H. Hulejova2, M. Braun2, J. Stork3 1outpatient department; 2connective tissue lab, Institute of Rheumatology; 3Clinic of Dermatovenerology, General Teaching Hospital, Praha 2, Czech Republic
Background: Systemic (SSc) and localized scleroderma (LSc) do not have reliable activity markers. An excessive deposition of collagen and elastin fibers occurs in both conditions.
Objectives: The aim of this study was to assess the degradation of collagen type I, elastin excretion and proinflammatory cytokines in systemic and localized scleroderma compared with psoriasis and healthy controls (HC).
Methods: Total 91 individuals were examined – 24 with systemic sclerosis (SSc), 16 with localized scleroderma (LSc), and 37 patients with psoriasis vulgaris (PsV) and 14 healthy donors as control groups. Urinary excretion of pyridinoline (U-PD) and deoxypyridinoline (U-DPD) were measured using sensitive isocratic HPLC method. Urinary exctretion of soluble elastin, serum levels of interleukin-6 (IL-6) and and soluble interleukin-2 receptor (IL-2R) were assayed using commercial ELISA kits. All measurements were performed at entry and after one year.
Results: U-PD levels were the highest at entry and after one year in SSc group. At entry U-PD concentrations in SSc and LSc groups were increased compared with HC (p 0.0001, and 0.0001 respectively). SSc patients had also a higher levels compared with PsV groups (p 0.001). At entry U-DPD levels were increased compared with HC and LSc (p 0.006, and p 0.001 respectively). Elastin excretion was the highest at entry in PsV and after one year in SSc group. At entry elastin concentrations in PsV group were increased compared with HC (p 0.001). After one year concentrations in SSc and PsV patients were higher compared with HC (p 0.001, and p 0.001 respectively). IL-6 serum levels were increased at entry in SSc, LSc and PsV groups compared with HC (p 0.0001, p 0.001, and p 0.004 respectively), after one year only SSc had increased levels compared with HC (p 0.001). IL-2r serum levels did not differ among the studied groups at any time.
Conclusion: Increased markers of collagen and elastin turnover in SSc and LSc reflect the active fibrotic process in the diseases and are accordance with the published data. High elastin levels in PsV group are rather difficult to explain.
This work was supported by the Ministry of Healthcare research grant No. 00000023728
Ann Rheum Dis 2008;67(Suppl II):362
Scleroderma, Myositis and related syndromes
|