[P-58] SUCCESS OF OOCYTE CRYOPRESERVATION IN NON-DONOR IVF CYCLES: A RANDOMIZED TRIAL OF SIBLING OOCYTES.
J. Kim, S. B. Ramos, M. A. Fritz, J. E. Mersereau. Obstetrics and Gynecology, University of North Carolina, Chapel Hill, NC.
OBJECTIVE: Prior studies have demonstrated comparable pregnancy outcomes in donor-egg IVF cycles using vitrified oocytes. Our aim was to investigate the laboratory and clinical outcomes using vitrified and then immediately thawed oocytes obtained from women in a range of age-groups undergoing IVF.
DESIGN: Prospective, randomized, clinical trial.
MATERIALS AND METHODS: Non-donor women undergoing IVF were enrolled in the study. Their oocytes were randomly assigned to either routine treatment or freezing with thaw 30-60 min later, and then all oocytes underwent ICSI fertilization at the same time. Only embryos from the freeze/thaw group were transferred. Laboratory outcomes (fertilization rates (FR), embryo quality) were compared among sibling oocytes.
RESULTS: Of the 8 enrolled women, the median age was 33 years. The median number of oocytes was 16 (range 12-33) and median maturation rate was 74%. The median thaw survival rate in the vitrified group was 80%. The FR and number of good quality day 3 embryos (>6 cells, no fragmentation) were not different between the fresh and vitrified groups. There were 4 clinical pregnancies (3 on-going and 1 missed abortion).
| Median (minimum, maximum) (n=8) | |||
| Age (years) | 33 (27, 36) | ||
| BMI (Kg/m2) | 25 (20, 32) | ||
| AMH (ng/ml) | 3.6 (1.1, 9.8) | ||
| Peak E2 level (pg/ml) | 3,328 (1,663, 4,989) | ||
| Total # of oocytes | 16 (12, 33) | ||
| No. of mature oocytes | 13 (8, 20) | ||
| Mturation rate (%) | 74 (57, 86) | ||
| Fresh group | Vitrified group | p-value† | |
| # of oocytes | 7 (4, 10) | 7 (4, 10) | |
| # of survuved oocytes after thawing | N/A | 5 (2, 7) | |
| Survival rate (%) | N/A | 80 (50, 100) | |
| Fertilization rate (%) | 85 (40, 90) | 75 (50, 100) | 1.00 |
| # of good quality embryos | 1 (0, 2) | 1 (0, 2) | 0.13 |