[P-58] SUCCESS OF OOCYTE CRYOPRESERVATION IN NON-DONOR IVF CYCLES: A RANDOMIZED TRIAL OF SIBLING OOCYTES.
J. Kim, S. B. Ramos, M. A. Fritz, J. E. Mersereau. Obstetrics and Gynecology, University of North Carolina, Chapel Hill, NC.
OBJECTIVE: Prior studies have demonstrated comparable pregnancy outcomes in donor-egg IVF cycles using vitrified oocytes. Our aim was to investigate the laboratory and clinical outcomes using vitrified and then immediately thawed oocytes obtained from women in a range of age-groups undergoing IVF.
DESIGN: Prospective, randomized, clinical trial.
MATERIALS AND METHODS: Non-donor women undergoing IVF were enrolled in the study. Their oocytes were randomly assigned to either routine treatment or freezing with thaw 30-60 min later, and then all oocytes underwent ICSI fertilization at the same time. Only embryos from the freeze/thaw group were transferred. Laboratory outcomes (fertilization rates (FR), embryo quality) were compared among sibling oocytes.
RESULTS: Of the 8 enrolled women, the median age was 33 years. The median number of oocytes was 16 (range 12-33) and median maturation rate was 74%. The median thaw survival rate in the vitrified group was 80%. The FR and number of good quality day 3 embryos (>6 cells, no fragmentation) were not different between the fresh and vitrified groups. There were 4 clinical pregnancies (3 on-going and 1 missed abortion).
|Median (minimum, maximum) (n=8)|
|Age (years)||33 (27, 36)|
|BMI (Kg/m2)||25 (20, 32)|
|AMH (ng/ml)||3.6 (1.1, 9.8)|
|Peak E2 level (pg/ml)||3,328 (1,663, 4,989)|
|Total # of oocytes||16 (12, 33)|
|No. of mature oocytes||13 (8, 20)|
|Mturation rate (%)||74 (57, 86)|
|Fresh group||Vitrified group||p-value†|
|# of oocytes||7 (4, 10)||7 (4, 10)|
|# of survuved oocytes after thawing||N/A||5 (2, 7)|
|Survival rate (%)||N/A||80 (50, 100)|
|Fertilization rate (%)||85 (40, 90)||75 (50, 100)||1.00|
|# of good quality embryos||1 (0, 2)||1 (0, 2)||0.13|