[O-239] CLINICAL EVALUATION OF THE FIRST ONE HUNDRED RECIPIENT CYCLES USING VITRIFIED/WARMED OOCYTES FROM A CRYO-EGG BANK AND COMPARISON OF OUTCOMES WITH FRESH OOCYTE DONATIONS.

Z. P. Nagy, C.-C. Chang, D. P. Bernal, A. A. Toledo, D. B. Shapiro, H. I. Kort Assisted Reproduction, Reproductive Biology Associates, Atlanta, GA

OBJECTIVE: To evaluate laboratory and clinical outcomes of the first 100 donor-recipient cycles, using vitrified donated oocytes, and compare them to 91 fresh oocyte donation cycles (where the donors for the cryo bank were the same as for the fresh donations).
DESIGN: Longitudinal observational study with retrospective data comparison.
MATERIALS AND METHODS: 81 oocyte donors (mean age: 26.4+/-2.7) performed 92 donation cycles for the cryo bank and another 91 cycles of fresh donations during the period of May 2006 and March 2009. A total of 1988 M2 eggs were vitrified for the donor-bank using a DMSO/EG vitrification protocol. ICSI was performed 2-3 h after warming (vitrified eggs) or 3-4 h after egg retrieval (fresh donations). Embryo transfer was performed on day 5. Results were evaluated using Chi-square or One-way ANOVA tests at P<0.05 level.
RESULTS: Mean ages of recipients were 40.9+/-4.9 and 41.2+/-4.7 (vitrified vs. fresh eggs respectively, NS). 604 out of 707 warmed oocytes survived the vitrification (85%) and were inseminated (mean 6.0 per patient); with fresh donation an average of 25.2 cumulus/egg complexes were used per patient (P<0.001). Fertilization rate was 76.5% and 56.8% in the vitrified/warmed vs. fresh donation groups respectively (P<0.01). Embryo developmental rates were similar on day 3 and day 5 in both groups. 2.08+/-0.54 and 1.97+/-0.51 embryos were transferred (NS) and 1.5+/-1.5 and 12.5+/-8.8 embryos were cryopreserved (P<0.0001) in the vitrified/warmed vs. fresh donation groups, respectively. Clinical pregnancy rate was 67% (67/100) and 69% (63/91; NS) and implantation rate was 52% (108/208) and 56% (100/179; NS) in the cryo vs. fresh donation groups, respectively.
CONCLUSIONS: The results of the present study demonstrate that cryo banking of donated oocytes provides excellent laboratory and clinical outcomes, comparable in all aspects to fresh donation (using the same donors); however, it represents a more efficient and possibly safer alternative both to the patients and to the practitioners.

Wednesday, October 21, 2009 4:00 PM

Oral Presentation: Cryopreservation and Frozen Embryo Transfer: ART